Title: Coffee (coffea arabica L.) seed germination after treatment with
different concentrations and embedding times in cellulase.
Authors: Sales, Juliana De Fatima; De Alvarenga, Amauri Alves; De Oliveira,
Joao; Nogueira, Francisco Dias; Rezende, Lucio Costa; Silva, Fabiano Guimaraes.
Authoes affiliation: Fisiologia Vegetal-UNIVERSIDADE FEDERAL DE LAVRAS/UFLA,
Lavras, Brazil.
Published in: Ciencia e Agrotecnologia , volumn 27, pages 557-564, (2003).
Abstract: "The seeds of coffee trees germinate slowly, because the seeding
is done soon after the crop is harvested, which coincides with the cold period
of the year, and also due to the parchment, which constitutes a barrier that
hinders the absorption of water for the seed. Aiming to evaluate the effect of
exogenous application of cellulase enzyme to coffee seed germination, an experiment
was performed soaking coffee seeds, cultivar "Acaia do Cen-ado". in
cellulase solution at different concentrations (0, 1.6, 3.2, and 6.4, g L-1) in
0.05 M K-citrate buffer at pH 4.8 for 0.72 and 144 hours. It was found that the
cellulase enzyme at the concentration of 6.4 g L-1 provided the greatest index
of speed germination and the greatest germination percentage when the seeds
remained in contact with the solution. for 144 hours."